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743 crrna targeting ist2 codons  (Addgene inc)


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    Structured Review

    Addgene inc 743 crrna targeting ist2 codons
    Fig. 1. <t>Ist2</t> is required for Osh6 localization to the cortical ER. (A) Localization of
    743 Crrna Targeting Ist2 Codons, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/743 crrna targeting ist2 codons/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    743 crrna targeting ist2 codons - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast."

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    Journal: Journal of cell science

    doi: 10.1242/jcs.243733

    Fig. 1. Ist2 is required for Osh6 localization to the cortical ER. (A) Localization of
    Figure Legend Snippet: Fig. 1. Ist2 is required for Osh6 localization to the cortical ER. (A) Localization of

    Techniques Used:

    Fig. 2. Osh6 interacts with the disordered cytosolic tail of Ist2. (A) Schematic representation
    Figure Legend Snippet: Fig. 2. Osh6 interacts with the disordered cytosolic tail of Ist2. (A) Schematic representation

    Techniques Used:

    Fig. 4. Osh6 binding site in Ist2 tail is sufficient for localizing Osh6 to the ER-PM contact sites.
    Figure Legend Snippet: Fig. 4. Osh6 binding site in Ist2 tail is sufficient for localizing Osh6 to the ER-PM contact sites.

    Techniques Used: Binding Assay

    Fig. 5. Mutations in Ist2 tail decrease steady-state levels of PS. Lipidomic analysis of WT,
    Figure Legend Snippet: Fig. 5. Mutations in Ist2 tail decrease steady-state levels of PS. Lipidomic analysis of WT,

    Techniques Used:

    Fig. 6. Binding of Osh6 to the disordered tail of Ist2 is required for PS transport to the PM.
    Figure Legend Snippet: Fig. 6. Binding of Osh6 to the disordered tail of Ist2 is required for PS transport to the PM.

    Techniques Used: Binding Assay

    Fig. 7. Identification of a conserved patch on Osh6 that interact with Ist2-tail. (A) Structure
    Figure Legend Snippet: Fig. 7. Identification of a conserved patch on Osh6 that interact with Ist2-tail. (A) Structure

    Techniques Used:

    Fig. 8. Osh6 D141/L142 mutants decrease interaction with Ist2 and PS transport. (A)
    Figure Legend Snippet: Fig. 8. Osh6 D141/L142 mutants decrease interaction with Ist2 and PS transport. (A)

    Techniques Used:



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    743 crrna targeting ist2 codons  (Addgene inc)
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    Addgene inc 743 crrna targeting ist2 codons
    Fig. 1. <t>Ist2</t> is required for Osh6 localization to the cortical ER. (A) Localization of
    743 Crrna Targeting Ist2 Codons, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/743 crrna targeting ist2 codons/product/Addgene inc
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    Fig. 1. <t>Ist2</t> is required for Osh6 localization to the cortical ER. (A) Localization of
    Prk5 Ha Sestrin2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fig. 1. Ist2 is required for Osh6 localization to the cortical ER. (A) Localization of

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 1. Ist2 is required for Osh6 localization to the cortical ER. (A) Localization of

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques:

    Fig. 2. Osh6 interacts with the disordered cytosolic tail of Ist2. (A) Schematic representation

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 2. Osh6 interacts with the disordered cytosolic tail of Ist2. (A) Schematic representation

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques:

    Fig. 4. Osh6 binding site in Ist2 tail is sufficient for localizing Osh6 to the ER-PM contact sites.

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 4. Osh6 binding site in Ist2 tail is sufficient for localizing Osh6 to the ER-PM contact sites.

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques: Binding Assay

    Fig. 5. Mutations in Ist2 tail decrease steady-state levels of PS. Lipidomic analysis of WT,

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 5. Mutations in Ist2 tail decrease steady-state levels of PS. Lipidomic analysis of WT,

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques:

    Fig. 6. Binding of Osh6 to the disordered tail of Ist2 is required for PS transport to the PM.

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 6. Binding of Osh6 to the disordered tail of Ist2 is required for PS transport to the PM.

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques: Binding Assay

    Fig. 7. Identification of a conserved patch on Osh6 that interact with Ist2-tail. (A) Structure

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 7. Identification of a conserved patch on Osh6 that interact with Ist2-tail. (A) Structure

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques:

    Fig. 8. Osh6 D141/L142 mutants decrease interaction with Ist2 and PS transport. (A)

    Journal: Journal of cell science

    Article Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    doi: 10.1242/jcs.243733

    Figure Lengend Snippet: Fig. 8. Osh6 D141/L142 mutants decrease interaction with Ist2 and PS transport. (A)

    Article Snippet: Maeda et al., 2013 pAC100 pADH1 OSH6-mCherry (U) ADH1prOSH6-mCherry, CEN, URA3 (pRS315-based); marker swap This study pVA111 pCYC1 Osh6-mCherry CYC1prOSH6-mCherry, CEN, URA3 (pRS316-based); OSH6 subcloned This study pJMD_22 pADH1prOsh6 L141A D142A Quikchange mutagenesis of pAC100 This study pJMD_23 pCYC1prOsh6 L141A D142A Quikchange mutagenesis of pVA111 This study pJMD_24 pADH1prOsh6 L141K D142A Quikchange mutagenesis of pAC100 This study pJMD_25 pCYC1 Osh6 L141K D142A Quikchange mutagenesis of pVA111 This study pC2Lact-GFP C2lact-GFP GPDprC2Lact-GFP, CEN, URA3 (pRS416-based) Yeung et al., 2008 pAC107 C2lact-GFP (LEU) GPDprC2Lact-GFP, CEN, LEU2 (pRS416-based) Lipp et al., 2019 pJMD_26 crRNA Ist2 736-743 crRNA targeting IST2 codons 736-743, URA, 2 μ ori, based on pUD628 (Addgene Plasmid #103018) This study pUDC175 fnCpf1 TEF1prFnCPF1, CEN, NatMX (p414TEF1 Backbone) Addgene Plasmid #103019 Swiat et al., 2017 pVA117 pRS415-ADHprOsh6D35-VN ADHprOsh6D35 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study pVA119 pRS415-ADHprOsh6D69-VN ADHprOsh6D69 (Lipp et al., 2019) subcloned into pRS415-VN using SacI/BamHI digestion This study J.

    Techniques: